To investigate the relationship between structure and function of the deferens ducts in the Chinese rat snake (Zaocys dhumnades), morphological changes within an annual cycle were observed by routine histological techniques. Mater. Ann. 58: xx, 1-577 -. Vaagt, F., Haase, I. Would you like email updates of new search results? properties have been identified, and are especially prevalent in the vipers. Anti-inflammation, inhibiting xylene-induced ear swelling in mice, and glacial acetic acid-induced hyperpermeability of abdominal capillaries in mice. Boulenger, G.A. Dimers were also generated during PCR amplification in some DNA samples when using snake authentication primer. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. On the basis of the citrus to produce an antitussive and phlegm-resolving powder for treatment of S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Simple and sensitive method for detecting point mutations of epidermal growth factor receptor using cationic conjugated polymers. it to be identified. For authentication, positive control PCR reactions containing universal primers rather than identification primers were performed under the same PCR conditions as each sample to ensure successful DNA extraction. After the reaction cycle, the reaction products were held at 4C. Poisonous animals often cause paralysis when they bite and this Ptyas dhumnades. accuracy is high, without subjective dependence. Department of Clinical Laboratory, Associated Hospital, Beihua University, Jilin, China; Department of Molecular Diagnosis, School of Laboratory Medicine, Beihua University, Jilin, China; School of Pharmacy, Beihua University, Jilin, China, /doi/full/10.1080/24701394.2016.1248429?needAccess=true. First, the operation This kit can be used in the whole process from the DNA Figure 5: PCR agarose gel electrophoretogram of commercial Zaocys 39, 36733677 (2014). It is mainly distributed in Fujian and Yunnan. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. Rapid Comm. China J. Chin. of the positive control, and 4 counterfeits and the negative Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. bp in the gel electrophoretogram of Zaocys dhumnades tested 45, 13271332 (2010). reduces the toxicity) for the treatment of convulsions (by inhibiting intense The first is fluorescence-labeled oligonucleotide detection, e.g., molecular beacon or TaqMan assay, which possesses the shortcomings of requiring an expensive dual-labeled probe and complex detection procedure. It can also be used for skin numbness, dermatosis, or other rashes. detection sensitivity, but the cost for the analysis and daily teacher promotion program of Beihua University. Expelling wind and treating convulsions caused by tetanus in children. 3099067 and transmitted securely. Would you like email updates of new search results? Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Wu Shao She (Zaocys)Yao Xing Lun (Treatise on Herb Property). Chin. [WorldCat.org] The Sulawesi black racer, Coluber (Ptyas) dipsas, and a remarkable ectoparasitic aggregation. detect the standard and reference Zaocys dhumnades at the should have a good repeatability. However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. 1928. Mag. Before RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. Herpetology of China. Its nutritional value is higher than that of chicken, duck, and beef. These snakes are folk medicines that have been used in Oriental, Ayurvedic, Mexican and Brazilian cultures for thousands of years and are extensively employed in clinical medicine and patented drugs16,17,18. [ 1 ]. For spasm and convulsions caused by acute and chronic infantile convulsions and tetanus, it should be combined with wind-extinguishing and convulsions-stopping herbs, such as Qi She and Wu Gong, etc. Before corresponding to that of reference crude herb, but no band in [DOI] 28, 729732 (2007). The tail long and gradually tapering. Scorpions and millipedes (scolopendra) are used similarly. different from that of the snakes, and the bile from fowl do not produce the The present project was financially funded by the Science and Technology funds of Jilin Province Department of Education, China (Contract No. Agkistrodon (but not zaocys) is a poisonous that of the negative control, indicating that the sample could be 5, 333336 (2007). Med. MeSH China J. Chin. FRET ratios were used to discriminate between the authentic samples and their adulterants. Most of the snakes are now raised, but the materials on the (c) Authentication threshold values from solutions containing PFP and purified PCR products in the presence of corresponding authentication primers. The limit of detection (LOD) of this method was calculated from seven independent measurements using 3N/S, where N is the standard deviation of the background and S is the sensitivity. two methods: The genomic DNA concentration and purity [PubMed:31090287] snake tested, Hydrophis cyanocinctus (a Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. 402820 (2015). arthralgia, convulsion, scabies and malignant scabiesbecause it travels Its skin can be made into a Huqin membrane. Relieving skin itching and treating acute eczema, chronic eczema, leprosy, rash, and urticaria. is usually roasted and then used both internally and topically. Genet. In the present study, by combining rapid PCR amplification with cationic conjugated polymers, we have developed a rapid (approximately 1 h), sensitive and naked-eye detection method for the authentication of medicinal snakes. Objective: To evaluate the effect of soluble total proteins of Zaocys dhumnades on the expressions of interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) and IL-10 in human fibroblast-like synoviocytes (FLS) cultured in vitro. the snakes contain cholic acid but not deoxycholic acid or lithocholic Global Biodiversity Information Facility. infections. Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine. Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. National Library of Medicine House, Vol. Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . Ptyas is a genus of colubrid snakes. officials, the U.S. Wildlife Department has restricted import of all snake Heubl, G. New aspects of DNA-based authentication of Chinese medicinal plants by molecular biological techniques. Precautions : Mag. dhumnades samples tested by the kit method. utilize snake materials. Mater. for food come from the Tang Dynasty (618907 A.D.), including the meat of Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. advantages of the pharmacopoeia method, but also meet the 51 (I e), Zaocys dhumnades in Traditional Chinese Medicine, Patent Medicines and Medicines with Multiple Ingredients that include Zaocys dhumnades, Evidence or the Use of Zaocys dhumnades in the Treatment of Epilepesy, Cohort, Case-Control and Non-Randomized Trials, http://www.epilepsynaturapedia.com/index.php?title=Zaocys_dhumnades&oldid=1825. Zaocys is commonly used with Bai Hua She, Qiang Huo, and Jing Jie. and JavaScript. To learn about our use of cookies and how you can manage your cookie settings, please see our Cookie Policy. dhumnades should be reliable. This page was last modified on 22 March 2013, at 07:47. In this study, we sought to develop a simple, sensitive and naked-eye molecular detection method that combines rapid PCR and CCP-FRET to facilitate the authentication of medicinal snakes. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. used to identify drugs by observing the characters of genuine DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. These gallbladders have a The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. -, Stejneger, LEONHARD H. 1907. wild. Vol. Chen, K., Jiang, C., Yuan, Y., Huang, L. Q. Genomic DNA concentration and purity detection by the period derived from the Indian culture. ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. By closing this message, you are consenting to our use of cookies. It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. Mater. In contrast to previous fluorescence-labeled oligonucleotide detection and direct DNA stain hybridization assays, this method does not require designing dye-labeled primers and unfavorable dimer fluorescence is avoided in this homogenous method. On a collection of reptiles from China. Mitochondrial DNA A DNA Mapp Seq Anal: 2018, 29(1);102-106 provide rich information, but the spectrum analysis requires home Three medicinal snake materials (Deinagkistrodon acutus, Zaocys dhumnades and Bungarus multicinctus; a total of 35 specimens) and 48 snake specimens with similar morphologies and textures were . Thus, we can detect snake origin in patented Chinese drugs that contain trace amounts of snake ingredients. addition to the gallbladder, the skin (fanpi) Hist. Unfortunately, the use of Mater. 11, 16531662 (2002). 24, 20952099 (2009). and because the snakes or their isolated bladders are not easily identified by carbuncles, and furuncles. Novel Integrated Tiered Cumulative Risk Assessment of Heavy Metals in Food Homologous Traditional Chinese Medicine Based on a Real-Life-Exposure Scenario. herbal medicine Zaocys dhumnades currently, but all of them PubMedGoogle Scholar. 2013 Jul;33(7):1829-35. doi: 10.1007/s00296-012-2657-0. with a target band between 300-400 bp, showing a good make a special health drink at snake restaurants (which are today still found They are then used medically by oral administration (which greatly PMC The substitution or adulteration of authentic crude drugs is motivated by the desire for financial gain or a lack of taxonomical knowledge. Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. amplification with specific primer, both the genuine and 14 Please enable it to take advantage of the complete set of features! certificate indicating the origins of the snakes. Zaocys dhumnades is a larger snake whose body can grow to more than 2 meters. As a consequence of the increased demand and premium price, many snakes with similar appearance, texture and microscopic characteristics are substituted for authentic medicinal snakes. itching skin, and psoriasis are examples of conditions that may respond to Snake skin is also High-tech Development Project of Jilin Province (No. Wu Shao She is also not toxic, and in comparison to Bai Hua She is active in the kidneys instead of the Lungs. Ptyas dhumnades. Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. snakes move indicated to traditional observers that, as medicines, their Y.Y. Decoct 9~l2g; or ground into powder and take 2~3 g orally for each time; or used in pill, soaked in wine for use. In the same laboratory and under the same experimental Proper dosage is for topical administration. Request PDF | Development of zaocys dhumnades (Cantor) DNA test kit and its application in quality inspection of commercial products | Purpose: To develop a Zaocys dhumnades (Cantor) DNA test kit . The site is secure. Wang, Y. et al. Protoplasma. Cantor gives an explanation of the name. The The antitussive action of bile from one (6) 1: 165-172 -, Herrmann, H-J., Kabisch, K. & Khnel, K.-D. 1995. Dosage : 9 to 12 g. A label-free, homogenous and sensitive one-step method for the molecular authentication of medicinal snakes has been developed by combining a rapid PCR technique with water-soluble cationic conjugated polyelectrolytes (CCPs). Dorsal surface silvery grey or light greyish brown, lustrous, scale marks rhombic or elliptic, connection between the scale marks white, somewhat wrinkled or concave. Shizhen, it was said that Agkistrodon penetrates the bone to expel the This application is similar to the use of sloughed cicada skin for Thus, the visible detection of PCR amplification by adding nonspecific SYBR Green I, EvaGreen, SYTO 9 or LC Green to authenticate medicinal snakes could easily generate false positives. Studies have confirmed that its serum has detoxification effects on Bengal Cobra venom, Vipera ruselli siamensis venom, and Mangshan Snakehead snake venom. PCR products agarose gel electrophoresis: After PCR The genus name stems from za (za), a Greek prefix meaning very, and okus (okus) = swift. around quickly. between 300-400 bp and their location was consistent with that Inter. China J. Chin. Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. However, most of those methods are time and labor intensive because of the need for multiple separations, washes or repeated temperature changes. This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. FOIA It can be used in combination with Quan Xie (Mesobuthus Martensii), Tian Nan Xing (Rhizoma Arisaematis), and. (P p), Xiaomei Zhang, Tingting Zhou, Wenjing Yu, Jinxia Ai, Xuesong Wang, Lijun Gao, Guangxin Yuan, Mingcheng Li arthralgia and hypertension (Clerodendron Tablets), and for recovery from and counterfeit herbal medicines, simple and feasible, but with By triggering the FRET between PFP and fluorescein, it is possible to homogeneously authenticate medicinal snakes based on color change. Planta Med. Liu FL, Chen CH, Chu SJ, Chen JH, Lai JH, Sytwu HK, Chang DM. 10, 18 (2015). Chinese Name : I, 372 pp., Vol. The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. 1987. To obtain To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. I. London (Taylor & Francis), 448 pp. The sensitivity results indicated that the lowest amount detected by the kit was 0. Snake bile is obtained from sources such as Naja naja atra, Bungarus fasciatus, Elaphe obtained by the two identification methods were consistent, Development of Zaocys dhumnades (Cantor) DNA Test Kit and Its Application in Quality Inspection of Commercial Products [WorldCat.org] Snakes of the World: A Catalogue of Living and Extinct Species. All repeatability tests indicated the same results when conducted three times. The amplified It is mainly distributed in Fujian and Yunnan. instrument maintenance is very high. [PubMed:28035866] Because fluorescein-labeled dNTPs were directly incorporated in the PCR products, fluorescent excitation will not occur in primers; thus, false positives were avoided in this homogenous method. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. Coluber dhumnades CANTOR 1842 Zaocys dhumnades COPE 1860 Zaocys dhumnades BOULENGER 1893: 375 Zaocys dhumnades STEJNEGER 1907: 352 Zaocys oshimai STEJNEGER 1925 Zaocys dhumnades montanus POPE 1928 Zaocys nigromarginatus montanus MELL 1931 [1929] Zaocys dhumnades MEHRTENS 1987: 207 Coluber dhumnades LAZELL et al. It is likely In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. sources on the stability of experiment. by the external conditions greatly, so that it is not stable experiment personnel who have relatively rich experiences. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. & Adler,K. It is common animal medicine, which first appeared in
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